Result : A0QSB6
| Protein Information |
| Information Type | Description |
|---|---|
| Protein name | Mycofactocin precursor peptide |
| NCBI Accession ID | |
| Organism | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Left | |
| Right | |
| Strand | |
| Nucleotide Sequence | |
| Sequence | MEPNQHVEAETELVTETLVEEVSIDGMCGVY |
| Source of smORF | Swiss-Prot |
| Function | Precursor peptide that leads to mycofactocin (MFT) after extensive post-translational modifications by enzymes encoded by adjacent genes. Mycofactocin acts as a redox cofactor of nicotinamide-dependent oxidoreductases encoded in the same locus (Pubmed:27158836). Is required for the in vivo ethanol assimilation in M.smegmatis (Pubmed:31113891). {ECO:0000269|Pubmed:27158836, ECO:0000269|Pubmed:31113891}. |
| Pubmed ID | 27158836 28077628 30183269 31113891 |
| Domain | CDD:274887 |
| Functional Category | Others |
| Uniprot ID | A0QSB6 |
| ORF Length (Amino Acid) | 31 |
++ More..
| Conservation Analysis |
No. of Species: 76
| Sr.No. | Left Position | Right Position | Strand | NCBI Accession id | Species Name |
|---|---|---|---|---|---|
| 1 | 3766300 | 3766395 | - | NZ_AP022617.1 | Mycolicibacterium monacense |
| 2 | 1597883 | 1597978 | + | NZ_LN831039.1 | Mycolicibacterium smegmatis |
| 3 | 6789116 | 6789211 | - | NZ_CP012150.1 | Mycobacterium goodii |
| 4 | 3291358 | 3291453 | - | NZ_AP022586.1 | Mycolicibacterium litorale |
| 5 | 4385997 | 4386092 | + | NZ_AP022567.1 | Mycolicibacterium mageritense |
| 6 | 5903825 | 5903920 | - | NZ_CP020809.1 | Mycobacterium dioxanotrophicus |
| 7 | 1181833 | 1181928 | + | NZ_AP022610.1 | Mycolicibacterium madagascariense |
| 8 | 4709162 | 4709257 | - | NZ_AP022561.1 | Mycolicibacterium aichiense |
| 9 | 4472811 | 4472906 | + | NZ_AP022569.1 | Mycobacterium cookii |
| 10 | 582369 | 582464 | - | NZ_AP022606.1 | Mycobacterium branderi |
| 11 | 4727648 | 4727743 | - | NZ_CP062008.1 | Mycolicibacterium mucogenicum DSM 44124 |
| 12 | 5019243 | 5019338 | + | NZ_AP022616.1 | Mycolicibacterium phocaicum |
| 13 | 1112443 | 1112535 | + | NZ_LR134356.1 | Mycolicibacterium aurum |
| 14 | 1139116 | 1139208 | - | NZ_AP022599.1 | Mycolicibacterium pulveris |
| 15 | 6463109 | 6463204 | - | NZ_AP022579.1 | Mycolicibacterium boenickei |
| 16 | 1463889 | 1463984 | + | NZ_CP011269.1 | Mycolicibacterium fortuitum |
| 17 | 5548929 | 5549021 | - | NZ_AP022574.1 | Mycolicibacterium psychrotolerans |
| 18 | 5214403 | 5214498 | + | NZ_AP022620.1 | Mycolicibacterium anyangense |
| 19 | 1388278 | 1388370 | + | NC_008726.1 | Mycolicibacterium vanbaalenii PYR-1 |
| 20 | 2481982 | 2482077 | - | NZ_AP022565.1 | Mycolicibacterium alvei |
| 21 | 5494769 | 5494882 | - | NZ_AP022560.1 | Mycolicibacterium moriokaense |
| 22 | 2223286 | 2223393 | - | NZ_AP022577.1 | Mycolicibacterium aubagnense |
| 23 | 2715623 | 2715712 | - | NZ_AP022576.1 | Mycobacterium florentinum |
| 24 | 2854674 | 2854772 | - | NZ_AP022608.1 | Mycolicibacterium gadium |
| 25 | 1144642 | 1144734 | + | NZ_CP011491.1 | Mycolicibacterium vaccae 95051 |
| 26 | 985364 | 985453 | + | NZ_LT906483.1 | Mycolicibacterium thermoresistibile |
| 27 | 4399093 | 4399182 | - | NZ_CP009360.4 | Mycobacterium avium subsp. hominissuis |
| 28 | 2385680 | 2385772 | + | NZ_CP043474.1 | Mycobacterium grossiae |
| 29 | 4000193 | 4000288 | - | NZ_LR134355.1 | Mycolicibacterium chitae |
| 30 | 2996374 | 2996463 | - | NZ_AP022587.1 | Mycobacterium stomatepiae |
| 31 | 4294258 | 4294347 | + | NC_022663.1 | Mycobacterium kansasii ATCC 12478 |
| 32 | 1380824 | 1380913 | + | NZ_AP022618.1 | Mycolicibacterium insubricum |
| 33 | 4705158 | 4705247 | - | NC_016948.1 | Mycobacterium paraintracellulare |
| 34 | 1222679 | 1222768 | - | NZ_AP022615.1 | Mycobacterium heidelbergense |
| 35 | 3718827 | 3718922 | - | NZ_AP022581.1 | Mycobacterium lacus |
| 36 | 906885 | 906974 | + | NZ_AP018164.1 | Mycobacterium shigaense |
| 37 | 4691160 | 4691249 | + | NZ_AP022573.1 | Mycobacterium saskatchewanense |
| 38 | 4026705 | 4026800 | - | NZ_AP022612.1 | Mycolicibacterium confluentis |
| 39 | 291440 | 291532 | - | NZ_AP022598.1 | Mycolicibacterium parafortuitum |
| 40 | 3345508 | 3345603 | - | NZ_AP022614.1 | Mycobacterium parmense |
| 41 | 803574 | 803663 | + | NC_015848.1 | Mycobacterium canettii CIPT 140010059 |
| 42 | 791757 | 791846 | + | NC_000962.3 | Mycobacterium tuberculosis H37Rv |
| 43 | 5522785 | 5522883 | + | NZ_AP022601.1 | Mycobacterium gallinarum |
| 44 | 4009450 | 4009539 | - | NZ_AP024310.1 | Mycobacterium heckeshornense |
| 45 | 2702625 | 2702714 | + | NZ_AP022583.1 | Mycobacterium noviomagense |
| 46 | 2913848 | 2913943 | - | NZ_AP022609.1 | Mycolicibacter hiberniae |
| 47 | 754202 | 754291 | + | NZ_CP011883.2 | Mycobacterium haemophilum DSM 44634 |
| 48 | 2452148 | 2452231 | + | NZ_AP022588.1 | Mycolicibacterium sediminis |
| 49 | 3259392 | 3259487 | + | NZ_AP022600.1 | Mycolicibacterium tokaiense |
| 50 | 2230771 | 2230860 | - | NZ_AP022603.1 | Mycolicibacterium fallax |
| 51 | 4450900 | 4450989 | - | NZ_CP023147.1 | Mycobacterium marseillense |
| 52 | 1270086 | 1270178 | + | NZ_AP018410.1 | Mycobacterium pseudoshottsii JCM 15466 |
| 53 | 4971196 | 4971285 | + | NZ_AP022619.1 | Mycobacterium paraseoulense |
| 54 | 5171526 | 5171615 | + | NZ_AP022582.1 | Mycobacterium seoulense |
| 55 | 1649230 | 1649322 | + | NZ_AP022570.1 | Mycolicibacterium poriferae |
| 56 | 100810 | 100905 | - | NZ_AP022570.1 | Mycolicibacterium poriferae |
| 57 | 3539334 | 3539435 | - | NZ_LT906469.1 | Mycolicibacter terrae |
| 58 | 3558063 | 3558164 | - | NC_015576.1 | Mycolicibacter sinensis |
| 59 | 2924992 | 2925093 | + | NZ_AP022562.1 | Mycobacterium novum |
| 60 | 1072423 | 1072515 | - | NZ_AP022589.1 | Mycolicibacter minnesotensis |
| 61 | 983184 | 983273 | + | NZ_LR130759.1 | Mycobacterium basiliense |
| 62 | 2705368 | 2705460 | + | NZ_AP022572.1 | Mycobacterium shottsii |
| 63 | 2662067 | 2662159 | + | NZ_CP058277.1 | Mycobacterium marinum |
| 64 | 652118 | 652213 | + | NZ_CP044231.1 | Microbacterium caowuchunii |
| 65 | 252168 | 252263 | + | NZ_LR026975.1 | Mycolicibacterium hassiacum DSM 44199 |
| 66 | 4574939 | 4575034 | - | NZ_CP022295.1 | Nocardioides aromaticivorans |
| 67 | 5150386 | 5150481 | - | NZ_CP022295.1 | Nocardioides aromaticivorans |
| 68 | 3875057 | 3875152 | - | NZ_LT906450.1 | Rhodococcus rhodochrous |
| 69 | 4229479 | 4229574 | - | NZ_CP022208.1 | Rhodococcus pyridinivorans |
| 70 | 3947709 | 3947804 | + | NZ_LS483468.1 | Rhodococcus coprophilus |
| 71 | 5305601 | 5305693 | + | NZ_CP033972.1 | Gordonia insulae |
| 72 | 3413741 | 3413833 | - | NZ_CP011853.1 | Gordonia phthalatica |
| 73 | 3723840 | 3723935 | - | NZ_CP029146.1 | Rhodococcus ruber |
| 74 | 5035444 | 5035533 | + | NZ_CP009896.1 | Pimelobacter simplex |
| 75 | 3870151 | 3870249 | + | NC_008278.1 | Frankia alni ACN14a |
| 76 | 6528299 | 6528385 | - | NZ_AP018920.1 | Pseudonocardia autotrophica |
| 77 | 2412783 | 2412869 | - | NC_006361.1 | Nocardia farcinica IFM 10152 |
| 78 | 1204200 | 1204307 | - | NZ_CP061007.1 | Saccharopolyspora spinosa |
++ More..
| Neighborhood Conservation Analysis |
* Arrows marked in Genome Diagram shows ORFs; Multiple PFAMs can be mapped to a single ORF.
* 'Small ORF' represents the entry/query analyzed.
* Image generated using 'gggenes'(R-Package).
Neighborhood Representative Chosen(Species): NZ_AP022617.1
| Sr.No. | Domain | Co-occurrence Frequency | No. of species in which domain occurs with smORF | Median distance b/w smORF and domain bearing ORFs | Orientation relative to smORF | PFAM Information |
|---|---|---|---|---|---|---|
| 1 | PF13641.8 | 0.8 | 61 | 3561 | same-strand | Glycosyltransferase like family 2 |
| 2 | PF13632.8 | 0.78 | 59 | 3561 | same-strand | Glycosyl transferase family group 2 |
| 3 | PF13506.8 | 0.79 | 60 | 3560.0 | same-strand | Glycosyl transferase family 21 |
| 4 | PF02633.16 | 0.87 | 66 | 2866.0 | same-strand | Creatinine amidohydrolase |
| 5 | PF01070.20 | 0.96 | 73 | 1501 | same-strand | FMN-dependent dehydrogenase |
| 6 | PF04055.23 | 1.0 | 76 | 311 | same-strand | Radical SAM superfamily |
| 7 | PF13186.8 | 0.93 | 71 | 311 | same-strand | Iron-sulfur cluster-binding domain |
| 8 | PF17754.3 | 0.97 | 74 | 101.5 | opposite-strand | MftR C-terminal domain |
| 9 | PF00440.25 | 0.97 | 74 | 102 | opposite-strand | Bacterial regulatory proteins, tetR family |
| 10 | PF07992.16 | 0.75 | 57 | 2693.5 | same-strand | Pyridine nucleotide-disulphide oxidoreductase |
| 11 | PF00070.29 | 0.75 | 57 | 2677 | same-strand | Pyridine nucleotide-disulphide oxidoreductase |
| 12 | PF14759.8 | 0.71 | 54 | 2670.5 | same-strand | Reductase C-terminal |
++ More..