ProsmORF-pred
Result : Q9KYH3
Protein Information
Information Type Description
Protein name Chaplin-G (Chaplin-F)
NCBI Accession ID AL939113.1
Organism Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Left 219142
Right 219414
Strand +
Nucleotide Sequence ATGTCTCGCATCGCGAAGGCCGCGGGTGTCGCCCTGGGCACCGGAGCCGTGGTGCTCAGCGGCACCGGCATGGCCATGGCGGACGCGGGCGCGGCGGGCGCGGCCGTCGGCTCGCCCGGTGTCCTGTCCGGCAACGTCGTCCAGGTCCCGGTGCACGTGCCGGTCAACCTCTGCGGCAACACCATCGACGTGATCGGCCTGCTGAACCCGGCCTTCGGCAACGCCTGCGAAAACGGCGACGACGACGACAAGAGCGGCGGCTACGGCGGCTGA
Sequence MSRIAKAAGVALGTGAVVLSGTGMAMADAGAAGAAVGSPGVLSGNVVQVPVHVPVNLCGNTIDVIGLLNPAFGNACENGDDDDKSGGYGG
Source of smORF Swiss-Prot
Function One of 8 partially redundant surface-active proteins required for efficient formation of aerial mycelium; the short chaplins assemble into a hydrophobic, amyloidal fibrillar surface layer that envelopes and protects aerial hyphae and spores, presumably anchored to the long chaplins (Pubmed:12832396, Pubmed:12832397, Pubmed:15228525, Pubmed:17462011). Chaplins have an overlapping function with the surface-active SapB peptide; chaplins are essential on minimal medium while on rich medium both chaplins and SapB are required for efficient aerial hyphae formation (Pubmed:17462011). Chaplins are also involved in cell attachment to a hydrophobic surface (Pubmed:19682261). Forms amyloid fibrils in vitro probably composed of stacked beta-sheets, at low extracellular concentrations individually restores the ability to form aerial hyphae to a chaplin-deficient strain (Pubmed:21526199). A small chaplin extract (ChpD, ChpE, ChpF, ChpG and ChpH) self-assembles into 2 different amyloids; small fibrils at the air-water interface form an amiphipathic membrane that resembles spore-surface structures involved in aerial hyphae formation, and hydrophilic fibrils in solution that resemble the fibers that attach cells to a hydrophobic surface. At the air-water interface the hydrophilic surface is in contact with water (probably equivalent to the peptidoglycan layer), while the hydrophobic face is exposed to the air, making the surface of the aerial hyphae hydrophobic (Pubmed:24012833). A small chaplin extract applied to a chaplin-deficient strain restores aerial hyphae formation (Pubmed:12832396, Pubmed:12832397). The small chaplin extract forms an amyloid-like structure similar to that seen on the surface of cells without rodlets (rdlA-rdlB deletions), and is highly surface active, reducing surface tension from 72 to 26 mJ/m(2), which probably allows escape of hyphae from an aqueous environment into air (Pubmed:12832396). ChpF and ChpG are sufficient to restore the rodlet layer and hydrophobicity to a strain deleted for the other 6 chaplin genes (Pubmed:15228525). {ECO:0000269|Pubmed:12832396, ECO:0000269|Pubmed:12832397, ECO:0000269|Pubmed:15228525, ECO:0000269|Pubmed:17462011, ECO:0000269|Pubmed:19682261, ECO:0000269|Pubmed:21526199, ECO:0000269|Pubmed:24012833}.
Pubmed ID 12000953 12832396 12832397 15228525 17462011 19682261 21526199 24012833 24413917
Domain
Functional Category Others
Uniprot ID Q9KYH3
ORF Length (Amino Acid) 90
++ More..
Conservation Analysis
Conservation Analysis
No. of Species: 83
Sr.No. Left Position Right Position Strand NCBI Accession id Species Name
1 2561554 2561835 + NZ_AP023439.1 Streptomyces tuirus
2 2566068 2566307 - NZ_AP023439.1 Streptomyces tuirus
3 1458151 1458393 - NZ_AP023439.1 Streptomyces tuirus
4 6198317 6198583 - NZ_AP023440.1 Streptomyces glomeroaurantiacus
5 6193494 6193733 + NZ_AP023440.1 Streptomyces glomeroaurantiacus
6 7380256 7380501 + NZ_AP023440.1 Streptomyces glomeroaurantiacus
7 5971414 5971653 + NZ_CP045643.1 Streptomyces fagopyri
8 7116156 7116401 + NZ_CP045643.1 Streptomyces fagopyri
9 6589561 6589848 - NC_013929.1 Streptomyces scabiei 87.22
10 6584191 6584430 + NC_013929.1 Streptomyces scabiei 87.22
11 4817883 4818164 + NZ_CP023693.1 Streptomyces cinereoruber
12 4801483 4801716 + NZ_CP023693.1 Streptomyces cinereoruber
13 4814942 4815181 + NZ_CP023693.1 Streptomyces cinereoruber
14 3191024 3191314 + NZ_CP021978.1 Streptomyces hawaiiensis
15 2027177 2027419 - NZ_CP021978.1 Streptomyces hawaiiensis
16 2876848 2877156 + NZ_CP012382.1 Streptomyces ambofaciens ATCC 23877
17 2880897 2881136 - NZ_CP012382.1 Streptomyces ambofaciens ATCC 23877
18 3251706 3251987 + NZ_CP015098.1 Streptomyces qaidamensis
19 3269359 3269586 - NZ_CP015098.1 Streptomyces qaidamensis
20 3256114 3256353 - NZ_CP015098.1 Streptomyces qaidamensis
21 2080596 2080838 - NZ_CP015098.1 Streptomyces qaidamensis
22 2118505 2118795 + NZ_CP032229.1 Streptomyces seoulensis
23 6128383 6128619 + NZ_CP032229.1 Streptomyces seoulensis
24 2121520 2121759 - NZ_CP032229.1 Streptomyces seoulensis
25 1118676 1118909 - NZ_CP032229.1 Streptomyces seoulensis
26 3265542 3265811 + NZ_CP034279.1 Streptomyces ficellus
27 3262778 3263017 + NZ_CP034279.1 Streptomyces ficellus
28 2362684 2362950 + NZ_CP065253.1 Streptomyces clavuligerus
29 5749788 5750021 + NZ_CP065253.1 Streptomyces clavuligerus
30 6538627 6538890 - NZ_CP023699.1 Streptomyces kanamyceticus
31 6532864 6533103 + NZ_CP023699.1 Streptomyces kanamyceticus
32 7827223 7827471 + NZ_CP023699.1 Streptomyces kanamyceticus
33 6517373 6517606 + NZ_CP023699.1 Streptomyces kanamyceticus
34 607157 607438 + NC_010572.1 Streptomyces griseus subsp. griseus NBRC 13350
35 6824866 6825099 + NC_010572.1 Streptomyces griseus subsp. griseus NBRC 13350
36 1608401 1608634 - NC_010572.1 Streptomyces griseus subsp. griseus NBRC 13350
37 3360536 3360802 + NZ_CP022685.1 Streptomyces formicae
38 3381892 3382125 - NZ_CP022685.1 Streptomyces formicae
39 3366306 3366545 - NZ_CP022685.1 Streptomyces formicae
40 7861137 7861418 - NZ_CP020563.1 Kitasatospora albolonga
41 1190367 1190600 - NZ_CP020563.1 Kitasatospora albolonga
42 6873802 6874035 + NZ_CP020563.1 Kitasatospora albolonga
43 8521365 8521649 - NZ_CP071139.1 Streptomyces nojiriensis
44 2465303 2465536 - NZ_CP071139.1 Streptomyces nojiriensis
45 8448791 8449030 + NZ_CP071139.1 Streptomyces nojiriensis
46 1501594 1501827 - NZ_CP023703.1 Streptomyces galilaeus
47 2829665 2829895 - NZ_CP023703.1 Streptomyces galilaeus
48 2813319 2813558 - NZ_CP023703.1 Streptomyces galilaeus
49 1130629 1130862 - NZ_CP024957.1 Streptomyces cavourensis
50 7346124 7346405 - NZ_CP024957.1 Streptomyces cavourensis
51 6359637 6359870 + NZ_CP024957.1 Streptomyces cavourensis
52 1215755 1215988 - NZ_CP070242.1 Streptomyces californicus
53 7535263 7535547 - NZ_CP070242.1 Streptomyces californicus
54 6658880 6659113 + NZ_CP070242.1 Streptomyces californicus
55 1356016 1356249 - NC_021177.1 Streptomyces fulvissimus DSM 40593
56 7719339 7719620 - NC_021177.1 Streptomyces fulvissimus DSM 40593
57 6810558 6810791 + NC_021177.1 Streptomyces fulvissimus DSM 40593
58 1203853 1204086 - NZ_CP020570.1 Streptomyces violaceoruber
59 7580101 7580385 - NZ_CP020570.1 Streptomyces violaceoruber
60 6692820 6693053 + NZ_CP020570.1 Streptomyces violaceoruber
61 7294163 7294444 - NZ_CP013738.1 Streptomyces globisporus C-1027
62 6436117 6436350 + NZ_CP013738.1 Streptomyces globisporus C-1027
63 1420768 1421001 - NZ_CP013738.1 Streptomyces globisporus C-1027
64 6101582 6101821 + NZ_CP032427.1 Streptomyces griseorubiginosus
65 2930950 2931252 + NZ_CP063374.1 Streptomyces chromofuscus
66 1646510 1646743 - NZ_CP063374.1 Streptomyces chromofuscus
67 2935810 2936049 - NZ_CP063374.1 Streptomyces chromofuscus
68 2750199 2750438 + NZ_CP023407.1 Streptomyces fungicidicus
69 1714229 1714447 - NZ_CP023407.1 Streptomyces fungicidicus
70 440778 441011 - NZ_CP029254.1 Streptomyces spongiicola
71 111871 112101 + NZ_CP029254.1 Streptomyces spongiicola
72 3806898 3807137 + NZ_CP029254.1 Streptomyces spongiicola
73 3806489 3806728 + NZ_CP029254.1 Streptomyces spongiicola
74 546292 546525 - NZ_CP029188.1 Streptomyces tirandamycinicus
75 1168907 1169140 + NZ_CP029188.1 Streptomyces tirandamycinicus
76 1172394 1172633 + NZ_CP029188.1 Streptomyces tirandamycinicus
77 1171988 1172227 + NZ_CP029188.1 Streptomyces tirandamycinicus
78 134276 134506 + NZ_CP029188.1 Streptomyces tirandamycinicus
79 871649 871930 + NZ_CP023701.1 Streptomyces subrutilus
80 206352 206591 - NZ_CP023701.1 Streptomyces subrutilus
81 5634300 5634539 + NZ_CP034687.1 Streptomyces griseoviridis
82 5639402 5639683 - NZ_CP034687.1 Streptomyces griseoviridis
83 567759 567992 - NZ_CP009922.3 Streptomyces xiamenensis
84 877002 877247 - NZ_CP009922.3 Streptomyces xiamenensis
85 6402506 6402793 - NZ_CP022744.1 Streptomyces lincolnensis
86 7644229 7644474 + NZ_CP022744.1 Streptomyces lincolnensis
87 7832177 7832410 + NZ_CP022744.1 Streptomyces lincolnensis
88 6397234 6397473 + NZ_CP022744.1 Streptomyces lincolnensis
89 5571041 5571337 - NZ_CP051006.1 Streptomyces griseofuscus
90 6668660 6668893 + NZ_CP051006.1 Streptomyces griseofuscus
91 5566244 5566483 + NZ_CP051006.1 Streptomyces griseofuscus
92 628689 628931 - NZ_CP051006.1 Streptomyces griseofuscus
93 6528311 6528550 + NZ_CP051006.1 Streptomyces griseofuscus
94 4420378 4420659 + NZ_CP051486.1 Streptomyces pratensis
95 5570133 5570366 - NZ_CP051486.1 Streptomyces pratensis
96 1722214 1722459 - NZ_CP023695.1 Streptomyces alboniger
97 4216895 4217134 + NZ_CP023695.1 Streptomyces alboniger
98 796548 796781 - NZ_CP054938.1 Streptomyces harbinensis
99 2329777 2330016 - NZ_CP021080.1 Streptomyces pluripotens
100 995669 995902 - NZ_CP031194.1 Streptomyces paludis
101 8081891 8082157 - NZ_CP031194.1 Streptomyces paludis
102 3321838 3322065 - NC_021985.1 Streptomyces collinus Tu 365
103 6336773 6337006 + NZ_CP022310.1 Streptomyces calvus
104 6196025 6196270 + NZ_CP022310.1 Streptomyces calvus
105 5007728 5008000 - NZ_CP023690.1 Streptomyces spectabilis
106 4987817 4988050 + NZ_CP023690.1 Streptomyces spectabilis
107 2484000 2484236 - NZ_CP023690.1 Streptomyces spectabilis
108 5002429 5002668 + NZ_CP023690.1 Streptomyces spectabilis
109 8086615 8086848 - NZ_CP016279.1 Streptomyces griseochromogenes
110 8261279 8261524 - NZ_CP016279.1 Streptomyces griseochromogenes
111 2623032 2623319 - NZ_CP040752.1 Streptomyces rectiverticillatus
112 6303944 6304186 - NZ_CP040752.1 Streptomyces rectiverticillatus
113 2496623 2496898 + NZ_CP015866.1 Streptomyces parvulus
114 2500557 2500796 - NZ_CP015866.1 Streptomyces parvulus
115 1544955 1545200 - NZ_CP015866.1 Streptomyces parvulus
116 7309792 7310010 + NZ_CP023688.1 Streptomyces rimosus
117 1003583 1003822 + NZ_CP023688.1 Streptomyces rimosus
118 3693860 3694153 + NZ_CP047020.1 Streptomyces broussonetiae
119 3699142 3699381 - NZ_CP047020.1 Streptomyces broussonetiae
120 2552980 2553198 - NZ_CP047020.1 Streptomyces broussonetiae
121 7462983 7463225 + NC_016109.1 Kitasatospora setae KM-6054
122 3756879 3757121 + NC_016109.1 Kitasatospora setae KM-6054
123 3757211 3757498 + NC_016109.1 Kitasatospora setae KM-6054
124 2329715 2329933 - NZ_CP034539.1 Streptomyces cyaneochromogenes
125 7758074 7758319 + NC_003155.5 Streptomyces avermitilis MA-4680 = NBRC 14893
126 5724098 5724340 - NZ_CP030073.1 Streptomyces cadmiisoli
127 1548974 1549216 - NZ_CP029043.1 Streptomyces nigra
128 2372124 2372357 - NZ_CP020569.1 Streptomyces gilvosporeus
129 5217603 5217836 + NZ_CP020569.1 Streptomyces gilvosporeus
130 2537881 2538123 - NZ_CP020569.1 Streptomyces gilvosporeus
131 2923042 2923287 + NZ_CP063373.1 Streptomyces ferrugineus
132 4315388 4315627 + NZ_CP023698.1 Streptomyces viridifaciens
133 4314577 4314819 - NZ_CP023698.1 Streptomyces viridifaciens
134 2642145 2642384 - NZ_CP072827.1 Streptomyces mobaraensis NBRC 13819 = DSM 40847
135 6680738 6680977 - NZ_CP072827.1 Streptomyces mobaraensis NBRC 13819 = DSM 40847
136 3560157 3560396 - NZ_CP017316.1 Streptomyces rubrolavendulae
137 1071854 1072087 - NZ_CP017316.1 Streptomyces rubrolavendulae
138 3711413 3711652 - NZ_CP023696.1 Streptomyces fradiae ATCC 10745 = DSM 40063
139 1096698 1096931 - NZ_CP023696.1 Streptomyces fradiae ATCC 10745 = DSM 40063
140 3952931 3953173 - NZ_CP031264.1 Streptacidiphilus bronchialis
141 6476244 6476489 + NZ_CP031264.1 Streptacidiphilus bronchialis
142 4049771 4050052 + NZ_CP034463.1 Streptomyces aquilus
143 4054764 4055003 - NZ_CP034463.1 Streptomyces aquilus
144 199076 199315 + NZ_CP072931.1 Streptomyces auratus AGR0001
145 1675885 1676124 - NZ_CP072931.1 Streptomyces auratus AGR0001
146 1995691 1995924 - NZ_CP027306.1 Streptomyces atratus
147 3394209 3394478 + NZ_CP011340.1 Streptomyces pristinaespiralis
148 6652112 6652360 + NZ_CP011340.1 Streptomyces pristinaespiralis
149 3994409 3994651 - NZ_CP031455.1 Streptomyces olivoreticuli subsp. olivoreticuli
150 7940967 7941266 - NZ_CP031455.1 Streptomyces olivoreticuli subsp. olivoreticuli
151 7267100 7267384 - NZ_CP023702.1 Streptomyces nitrosporeus
152 6324043 6324276 + NZ_CP023702.1 Streptomyces nitrosporeus
153 579276 579554 - NZ_CP070326.1 Streptomyces noursei
154 2840197 2840439 - NZ_CP070326.1 Streptomyces noursei
155 5478834 5479085 + NZ_CP070326.1 Streptomyces noursei
156 2839788 2840033 - NZ_CP070326.1 Streptomyces noursei
157 5345047 5345325 + NZ_CP010407.1 Streptomyces vietnamensis
158 5340932 5341171 + NZ_CP010407.1 Streptomyces vietnamensis
159 2074683 2074925 - NZ_CP019457.1 Streptomyces lydicus
160 4880197 4880472 + NZ_CP029196.1 Streptomyces venezuelae
161 4876150 4876389 + NZ_CP029196.1 Streptomyces venezuelae
162 5670383 5670670 + NZ_CP059991.1 Streptomyces gardneri
163 5666890 5667129 + NZ_CP059991.1 Streptomyces gardneri
164 7516780 7517025 + NZ_CP017248.1 Streptomyces fodineus
165 7680704 7680937 + NZ_CP017248.1 Streptomyces fodineus
166 6817133 6817378 + NZ_CP026652.1 Streptomyces dengpaensis
167 7006110 7006343 + NZ_CP026652.1 Streptomyces dengpaensis
168 6025083 6025322 - NC_013131.1 Catenulispora acidiphila DSM 44928
169 114178 114417 + NZ_CP045096.1 Streptomyces phaeolivaceus
170 3411170 3411457 + NZ_CP045096.1 Streptomyces phaeolivaceus
171 3416453 3416692 - NZ_CP045096.1 Streptomyces phaeolivaceus
172 6834057 6834293 + NZ_CP023692.1 Streptomyces vinaceus
173 7290886 7291116 - NZ_CP023692.1 Streptomyces vinaceus
174 4335216 4335443 - NZ_CP023692.1 Streptomyces vinaceus
175 7084096 7084335 + NZ_CP060404.1 Streptomyces buecherae
176 2175255 2175497 - NZ_CP023694.1 Streptomyces coeruleorubidus
177 4830029 4830304 - NZ_CP032698.1 Streptomyces hundungensis
178 4824897 4825136 + NZ_CP032698.1 Streptomyces hundungensis
179 6775450 6775683 + NZ_CP032698.1 Streptomyces hundungensis
180 4949576 4949809 + NZ_CP023691.1 Streptomyces platensis
181 1402259 1402504 - NZ_CP042266.1 Streptomyces qinzhouensis
182 5045245 5045499 - NZ_CP042266.1 Streptomyces qinzhouensis
183 1580075 1580320 - NZ_CP020700.1 Streptomyces tsukubensis
184 5182462 5182716 - NZ_CP020700.1 Streptomyces tsukubensis
185 363691 363930 - NC_016111.1 Streptomyces cattleya NRRL 8057 = DSM 46488
186 7667616 7667861 + NZ_CP023689.1 Streptomyces chartreusis
187 5333367 5333621 + NZ_LN831790.1 Streptomyces leeuwenhoekii
188 6375538 6375771 + NZ_LN831790.1 Streptomyces leeuwenhoekii
189 2873036 2873290 - NZ_CP010849.1 Streptomyces cyaneogriseus subsp. noncyanogenus
190 1798935 1799168 - NZ_CP010849.1 Streptomyces cyaneogriseus subsp. noncyanogenus
++ More..
Neighborhood Conservation Analysis
* Arrows marked in Genome Diagram shows ORFs; Multiple PFAMs can be mapped to a single ORF.
* 'Small ORF' represents the entry/query analyzed.
* Image generated using 'gggenes'(R-Package).
Neighborhood Conservation Analysis
Neighborhood Representative Chosen(Species): NZ_CP045643.1
Sr.No. Domain Co-occurrence Frequency No. of species in which domain occurs with smORF Median distance b/w smORF and domain bearing ORFs Orientation relative to smORF PFAM Information
1 PF03777.15 0.66 55 202 same-strand ChpA-C
++ More..